Impact of Sperm DNA Fragmentation on Pregnancy Outcomes

Shweta  Dagar; Sandeep  Verma

doi:10.69968/ijisem.2025v4i3221-229

Authors

Shweta Dagar Embryologist, IVF, Sage University, Indore.
Sandeep Verma HOD Sage University Indore, Sage University, Indore.

DOI:

https://doi.org/10.69968/ijisem.2025v4i3221-229

Keywords:

Sperm DNA fragmentation, male infertility, oxidative stress, assisted reproduction, embryo quality

Abstract

This study aims to perform a meta-analysis in order to gain a thorough understanding and evaluation of the relationship between the sperm DNA Fragmentation Index (DFI) and the outcome of pregnancy following intracytoplasmic sperm injection (ICSI) or in vitro fertilisation (IVF) treatment. After receiving the approval from institutional review board for the use of human beings, sample were collected from the 88 couples who were ready to participate in the study. Initial IVF screening of patients were done. For that every female subject had a preliminary endocrine examination on Day 2 or the third day of their menstrual period. The PRL, FSH, LH, OESTRADIOL, and P4 were measured after taking the blood samples. For the ovulation induction long and short protocols were followed. The embryo quality was assessed. GnRH agonist and antagonist protocols” were employed for ovarian stimulation. Recombinant hMG and FSH were utilized to stimulate ovaries. The recovered oocytes were inseminated using either traditional insemination techniques, such as IVF, or micromanipulation methods like ICSI. Oocyte collection, identification, and assessment were done. Sperm concentration, motility, and morphology exhibit significant diversity, as revealed by descriptive statistics used in semen analysis. Using Pearson's chi-square test, the study's analysis reveals substantial correlations between clinical pregnancy outcomes and factors like embryo quality and prior therapies, pointing to areas that should be specifically targeted for improvement.

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