Formulation and evaluation of solid lipid nano particle of Vaccinium Macrocarpon Extract and Its Antimicrobial Activity

Diksha Gurjar; Brajesh Kumar Arjariya; Sheenam Mansuri

doi:10.69968/ijisem.2026v5i2431-438

Authors

Diksha Gurjar M. Pharma IV Sem (Pharmaceutics), Malhotra College of Pharmacy, Bhopal, M.P.
Brajesh Kumar Arjariya Professor & Head, Department of Pharmaceutics, Malhotra College of Pharmacy, Bhopal, M.P.
Sheenam Mansuri Associate Professor, Malhotra College of Pharmacy, Bhopal, M.P.

DOI:

https://doi.org/10.69968/ijisem.2026v5i2431-438

Keywords:

Solid Lipid Nanoparticles, Vaccinium Macrocarpon, Antimicrobial Activity, Solvent Evaporation Method, Phytochemical Screening, Zeta Potential

Abstract

A promising and adaptable drug delivery method that fills the gap between "polymeric nanoparticles" and "lipid emulsions" is solid lipid nanoparticles (SLNs). The goal of the current work was to formulate, optimize, and assess SLNs loaded with cranberry (Vaccinium macrocarpon) extract for increased antibacterial activity. Vaccinium macrocarpon, a member of the Ericaceae family, is renowned for its broad-spectrum antibacterial qualities, antioxidant potential, and high phenolic and flavonoid content. Methanol and petroleum ether were used as solvents in the soxhlet extraction process to create the extract. Flavonoids, alkaloids, tannins, glycosides, phenolic chemicals, triterpenoids, and steroids were all found in the methanolic extract, according to phytochemical screening. The results showed that the "total flavonoid content (TFC)" was 15.23 mg per gram equivalent of rutin and the total phenolic content (TPC) was 62.03 mg per gram equivalent of gallic acid. "Compritol" was used as the "lipid phase" and "sodium lauryl sulphate" as the "surfactant" in the solvent evaporation process utilized to create SLNs. Lipid and surfactant amounts were varied to create five formulations (F1–F5). Malvern Zetasizer's particle size study showed sizes ranging from 574.2 to 993.4 nm; formulation F3 had the smallest particle size, measuring 574.2 nm. The range of zeta potential readings was "-1.2 mV to 5.3 mV". The smooth surface and spherical shape were verified by scanning electron microscopy (SEM). The antimicrobial activity of optimized formulation "F3" against "Escherichia coli" was demonstrated by dose-dependent zones of inhibition measuring 7 mm, 10 mm, and 13 mm at 0.5, 1.0, and 1.5 mg/mL, respectfully. The F3 formulation remained stable for a three-month period with no appreciable changes in size of particles or zeta potential, according to stability assays conducted under accelerated settings. The findings show that SLNs loaded with Vaccinium macrocarpon extract are a stable and practical nanoformulation with strong antibacterial activity.

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